One critical group of plants that has been largely overlooked in the genomics revolution consists of economically significant agricultural weeds ( Stewart, 2009 Stewart et al., 2009). Furthermore, genome sequences of additional plant species will soon be available from in-progress large-scale sequencing projects ( ). From the model plant Arabidopsis ( Arabidopsis thaliana Arabidopsis Genome Initiative, 2000) to important crop plants such as rice ( Oryza sativa Goff et al., 2002 Yu et al., 2002 International Rice Genome Sequencing Project, 2005), soybean ( Glycine max Schmutz et al., 2010), maize ( Zea mays Schnable et al., 2009), and chickpea ( Cicer arietinum Varshney et al., 2013) to economic woody plants such as wine grape ( Vitis vinifera Jaillon et al., 2007) and poplar ( Populus trichocarpa Tuskan et al., 2006), powerful complete genome data sets and tools allow the unprecedented ability to explore the genetics and genomics of plant form and function. Complete or draft genome data are currently available for tens of plant species ( ). In the past few years, genomic approaches have revolutionized plant biology. To our knowledge, this article represents the first published draft genome of an agricultural weed. The genome will also be useful as another reference genome in the Compositae. The draft genome will be useful to better understand weediness and the evolution of herbicide resistance and to devise new management strategies. Genomic patterns were detected that associated with glyphosate-resistant or -susceptible biotypes. Simple sequence repeat and single-nucleotide polymorphisms were surveyed. These sequence data were assembled and used to analyze genome variation. Genome resequencing of seven additional horseweed biotypes was performed. The nuclear genome is composed of 44,592 protein-coding genes. The assembly covered 92.3% of the genome, including the complete chloroplast genome (approximately 153 kb) and a nearly complete mitochondrial genome (approximately 450 kb in 120 scaffolds). From 116.3 Gb (approximately 350× coverage) of data, the genome was assembled into 13,966 scaffolds with 50% of the assembly = 33,561 bp. We undertook a draft de novo genome assembly of horseweed by combining data from multiple sequencing platforms (454 GS-FLX, Illumina HiSeq 2000, and PacBio RS) using various libraries with different insertion sizes (approximately 350 bp, 600 bp, 3 kb, and 10 kb) of a Tennessee-accessed, glyphosate-resistant horseweed biotype. Thus, it is an appropriate candidate to help us understand the genetic and genomic bases of weediness. Horseweed, one of the most problematic weeds in the world, is a true diploid (2 n = 2 x = 18), with the smallest genome of any known agricultural weed (335 Mb). Customers who currently have, or later purchase, a QIAGEN CLC Microbial Genomics Module Server Extension, could also be provided the Microbial Genomics Module under the same terms.Horseweed ( Conyza canadensis), a member of the Compositae (Asteraceae) family, was the first broadleaf weed to evolve resistance to glyphosate.Customer with a QIAGEN CLC Genomics Server license agrees to act as the license administrator and distribute licenses to students.Registered users must be actively enrolled students, and registered for the course, subject to verification by QIAGEN and the sponsoring faculty member.Each educational license is limited to a 4-month subscription per course, or to the duration of the course (whichever is shorter).Registrants for the educational license to QIAGEN CLC Genomics Workbench Premium must hold an active QIAGEN CLC Genomics Server (master) license.Upon registration, licenses and direct download links will be provided to the professor/educator, who agrees to act as the 'educational training license administrator'. The student licenses can be issued repeatedly for the same educator, provided they are used for different courses.
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